EGLN1 antibody (C-Term)

Catalog No. ABIN2775907

The Rabbit Polyclonal anti-EGLN1 antibody has been validated for WB. It is suitable to detect EGLN1 in samples from Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for EGLN1 antibody (C-Term) (ABIN2775907)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGLN1 Antibody

Binding Specificity: C-Term

Sequence: QPAYATRYAI TVWYFDADER ARAKVKYLTG EKGVRVELNK PSDSVGKDVF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EGLN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGLN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 426 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background: EGLN1 has a role in the regulation of hypoxia-inducible factor. It is not affected by overexpression or downregulation of HIF-2alpha.
Alias Symbols: HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2
Protein Interaction Partner: FKBP5, UBC, HIF1A, IKBKG, ZNF281, KRAS, PKM, EPAS1, FKBP8, OS9, MAGEA11, MAGEA9, OSR1, SIAH2, LIMD1, VHL, SRC, SPRY2, EGLN3, EGLN1, HIST1H3A, ING4, HIF3A, POLR2A,
Protein Size: 426

Molecular Weight: 46 kDa

Gene ID: 54583

NCBI Accession: NM_022051, NP_071334

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect